Calculate GC content at synonymous position of codons (using four-fold degenerate sites only).
Usage
get_gc4d(cf, codon_table = get_codon_table(), level = "subfam")
Arguments
- cf
matrix of codon frequencies as calculated by
count_codons()
.- codon_table
a table of genetic code derived from
get_codon_table
orcreate_codon_table
.- level
"subfam" (default) or "amino_acid". For which level to determine GC contents at 4-fold degenerate sites.
Examples
# estimate GC4d of yeast genes
cf_all <- count_codons(yeast_cds)
gc4d <- get_gc4d(cf_all)
head(gc4d)
#> YPL071C YLL050C YMR172W YOR185C YLL032C YBR225W
#> 0.4047619 0.3114754 0.3652482 0.3372093 0.3259259 0.3304348
hist(gc4d)